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lncrna/mrna expression profile microarray  (Arraystar inc)

 
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    Arraystar inc lncrna/mrna expression profile microarray
    Lncrna/Mrna Expression Profile Microarray, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lncrna+expression+microarray/lncrna+mrna+expression+profile+microarray/pmc11470819-54-15-14
    Average 90 stars, based on 1 article reviews
    lncrna/mrna expression profile microarray - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Microarray:

    Article Title: N-3-(oxododecanoyl)-l-homoserine lactone suppresses dendritic cell maturation by upregulating the long noncoding RNA NRIR
    Article Snippet: XUAN ZHANG , YANG LIU, YANG LU, SONG LI, JIANPING LIU, YUNYAN ZHANG, LINA WANG, MO LI, YANFEN LUO, WEIZHENG ZHANG, CHA CHEN* and YOUQIANG LI* Department of Laboratory Medicine, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou 510006, People’s Republic of China The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510006, People’s Republic of China Guangzhou University of Chinese Medicine, Guangzhou 510006, People’s Republic of China Department of Stomatology, Guangzhou Women and Children’s Medical Center, Guangzhou 510623, People’s Republic of China Present Address: Department of Laboratory Medicine, The Affiliated Hexian Memorial Hospital of Southern Medical University, Guangzhou, People’s Republic of China

    Article Title: SATB2-AS1 Suppresses Colorectal Carcinoma Aggressiveness by Inhibiting SATB2-Dependent Snail Transcription and Epithelial–Mesenchymal Transition
    Article Snippet: .. Briefly, total RNA was extracted from tissues and used to synthesize double-stranded cDNA. cDNA was labeled and hybridized to the LncRNA Expression Microarray (Arraystar, Rockville, MD). .. After hybridization and washing, slides were scanned with the Axon GenePix 4000B microarray scanner (Molecular Devices, Sunnyvale, CA).

    Article Title: SEMA3B‐AS1 suppresses colorectal carcinoma progression by inhibiting Semaphorin 3B‐dependent VEGF signaling pathway activation
    Article Snippet: .. LncRNA Expression Microarray (Arraystar) was used to label, purify, and hybridize the cDNA. .. An Agilent Microarray Scanner (Agilent p/n G2565BA) was used to scan slides after hybridization and washing.

    Article Title: Piplartine suppresses proliferation and invasion of hepatocellular carcinoma by LINC01391-modulated Wnt/β-catenin pathway inactivation through ICAT.
    Article Snippet: Accepted Manuscript Piplartine suppresses proliferation and invasion of hepatocellular carcinoma by LINC01391-modulated Wnt/β-catenin pathway inactivation through ICAT Xiaoxi Fan, Jingjing Song, Zhongwei Zhao, Minjiang Chen, Jianfei Tu, Chenying Lu, Fazong Wu, Dengke Zhang, Qiaoyou Weng, Liyun Zheng, Min Xu, Jiansong Ji PII: S0304-3835(19)30369-6 DOI: https://doi.org/10.1016/j.canlet.2019.06.008 Reference: CAN 114437 To appear in: Cancer Letters Received Date: 11 February 2019 Revised Date: 10 June 2019 Accepted Date: 11 June 2019 Please cite this article as: X.. Fan, J.. Song, Z. Zhao, M. Chen, J. Tu, C. Lu, F. Wu, D. Zhang, Q. Weng, L. Zheng, M. Xu, J. Ji, Piplartine suppresses proliferation and invasion of hepatocellular carcinoma by LINC01391-modulated Wnt/β-catenin pathway inactivation through ICAT, Cancer Letters, https:// doi.org/10.1016/j.canlet.2019.06.008.

    Article Title: Down-Regulation of Lnc-CYP7A1-1 Rejuvenates Aged Human Mesenchymal Stem Cells to Improve Their Efficacy for Heart Repair Through SYNE1
    Article Snippet: .. Total RNA was isolated from hBM-MSCs after cultured in hypoxic conditions for 72 h. 12 × 135K lncRNA Expression Microarray (Arraystar, Rockville, MD, United States) was used to detected hBM-MSCs cDNA. .. After hybridization, the processed slides were scanned by the Axon GenePix 4000B microarray scanner.

    Article Title: Alterations in the long non-coding RNA transcriptome in mesangial cells treated with aldosterone in vitro
    Article Snippet: Cells were cultured in 6-well plates for 24 h. MCs were washed with PBS three times, then 200 μl EDTA (Gibco; Thermo Fisher Scientific, Inc.) was added to each well followed by 750 μl TRIzol ® reagent (Invitrogen; Thermo Fisher Scientific, Inc.). .. The cells were stored at −80°C and sent to the LncRNA Expression Microarray (Arraystar, Rockville, MD, USA). .. The lncRNAs were constructed using public transcriptome databases (Refseq, www.ncbi.nlm.nih.gov/refseq ; University of California Santa Cruz knowngenes, genome.ucsc.edu ; and Gencode, www.gencodegenes.org ), in addition to a publication ( ).

    Article Title: <p>Long Non-Coding RNA DLEU1 Up-Regulates BIRC6 Expression by Competitively Sponging miR-381-3p to Promote Cisplatin Resistance in Nasopharyngeal Carcinoma</p>
    Article Snippet: Protein was normalized with β-actin (Cell Signaling Technology). .. Microarray Analysis Total RNA was extracted from three NPC tissues and three normal nasopharyngeal epithelial tissues using TRIzol submit your manuscript | www.dovepress.com DovePress OncoTargets and Therapy 2020:132038 O nc oT ar ge ts a nd T he ra py d ow nl oa de d fr om h ttp s: //w w w .d ov ep re ss .c om / b y 94 .2 31 .2 19 .7 3 on 2 7- M ar -2 02 0 F or p er so na l u se o nl y. Powered by TCPDF (www.tcpdf.org) 1 / 1 Reagent (Invitrogen) and purified by an RNeasy Mini Kit (Qiagen, Valencia, CA). cDNAwas synthesized, labeled, and hybridized to the LncRNA Expression Microarray (Arraystar, Rockville, MD). ..

    Article Title: Long Non-Coding RNA DLEU1 Up-Regulates BIRC6 Expression by Competitively Sponging miR-381-3p to Promote Cisplatin Resistance in Nasopharyngeal Carcinoma
    Article Snippet: Protein was normalized with β-actin (Cell Signaling Technology). .. Total RNA was extracted from three NPC tissues and three normal nasopharyngeal epithelial tissues using TRIzol Reagent (Invitrogen) and purified by an RNeasy Mini Kit (Qiagen, Valencia, CA). cDNA was synthesized, labeled, and hybridized to the LncRNA Expression Microarray (Arraystar, Rockville, MD). ..

    Labeling:

    Article Title: N-3-(oxododecanoyl)-l-homoserine lactone suppresses dendritic cell maturation by upregulating the long noncoding RNA NRIR
    Article Snippet: XUAN ZHANG , YANG LIU, YANG LU, SONG LI, JIANPING LIU, YUNYAN ZHANG, LINA WANG, MO LI, YANFEN LUO, WEIZHENG ZHANG, CHA CHEN* and YOUQIANG LI* Department of Laboratory Medicine, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou 510006, People’s Republic of China The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510006, People’s Republic of China Guangzhou University of Chinese Medicine, Guangzhou 510006, People’s Republic of China Department of Stomatology, Guangzhou Women and Children’s Medical Center, Guangzhou 510623, People’s Republic of China Present Address: Department of Laboratory Medicine, The Affiliated Hexian Memorial Hospital of Southern Medical University, Guangzhou, People’s Republic of China

    Article Title: SATB2-AS1 Suppresses Colorectal Carcinoma Aggressiveness by Inhibiting SATB2-Dependent Snail Transcription and Epithelial–Mesenchymal Transition
    Article Snippet: .. Briefly, total RNA was extracted from tissues and used to synthesize double-stranded cDNA. cDNA was labeled and hybridized to the LncRNA Expression Microarray (Arraystar, Rockville, MD). .. After hybridization and washing, slides were scanned with the Axon GenePix 4000B microarray scanner (Molecular Devices, Sunnyvale, CA).

    Article Title: Piplartine suppresses proliferation and invasion of hepatocellular carcinoma by LINC01391-modulated Wnt/β-catenin pathway inactivation through ICAT.
    Article Snippet: Accepted Manuscript Piplartine suppresses proliferation and invasion of hepatocellular carcinoma by LINC01391-modulated Wnt/β-catenin pathway inactivation through ICAT Xiaoxi Fan, Jingjing Song, Zhongwei Zhao, Minjiang Chen, Jianfei Tu, Chenying Lu, Fazong Wu, Dengke Zhang, Qiaoyou Weng, Liyun Zheng, Min Xu, Jiansong Ji PII: S0304-3835(19)30369-6 DOI: https://doi.org/10.1016/j.canlet.2019.06.008 Reference: CAN 114437 To appear in: Cancer Letters Received Date: 11 February 2019 Revised Date: 10 June 2019 Accepted Date: 11 June 2019 Please cite this article as: X.. Fan, J.. Song, Z. Zhao, M. Chen, J. Tu, C. Lu, F. Wu, D. Zhang, Q. Weng, L. Zheng, M. Xu, J. Ji, Piplartine suppresses proliferation and invasion of hepatocellular carcinoma by LINC01391-modulated Wnt/β-catenin pathway inactivation through ICAT, Cancer Letters, https:// doi.org/10.1016/j.canlet.2019.06.008.

    Article Title: <p>Long Non-Coding RNA DLEU1 Up-Regulates BIRC6 Expression by Competitively Sponging miR-381-3p to Promote Cisplatin Resistance in Nasopharyngeal Carcinoma</p>
    Article Snippet: Protein was normalized with β-actin (Cell Signaling Technology). .. Microarray Analysis Total RNA was extracted from three NPC tissues and three normal nasopharyngeal epithelial tissues using TRIzol submit your manuscript | www.dovepress.com DovePress OncoTargets and Therapy 2020:132038 O nc oT ar ge ts a nd T he ra py d ow nl oa de d fr om h ttp s: //w w w .d ov ep re ss .c om / b y 94 .2 31 .2 19 .7 3 on 2 7- M ar -2 02 0 F or p er so na l u se o nl y. Powered by TCPDF (www.tcpdf.org) 1 / 1 Reagent (Invitrogen) and purified by an RNeasy Mini Kit (Qiagen, Valencia, CA). cDNAwas synthesized, labeled, and hybridized to the LncRNA Expression Microarray (Arraystar, Rockville, MD). ..

    Article Title: Long Non-Coding RNA DLEU1 Up-Regulates BIRC6 Expression by Competitively Sponging miR-381-3p to Promote Cisplatin Resistance in Nasopharyngeal Carcinoma
    Article Snippet: Protein was normalized with β-actin (Cell Signaling Technology). .. Total RNA was extracted from three NPC tissues and three normal nasopharyngeal epithelial tissues using TRIzol Reagent (Invitrogen) and purified by an RNeasy Mini Kit (Qiagen, Valencia, CA). cDNA was synthesized, labeled, and hybridized to the LncRNA Expression Microarray (Arraystar, Rockville, MD). ..

    Expressing:

    Article Title: N-3-(oxododecanoyl)-l-homoserine lactone suppresses dendritic cell maturation by upregulating the long noncoding RNA NRIR
    Article Snippet: XUAN ZHANG , YANG LIU, YANG LU, SONG LI, JIANPING LIU, YUNYAN ZHANG, LINA WANG, MO LI, YANFEN LUO, WEIZHENG ZHANG, CHA CHEN* and YOUQIANG LI* Department of Laboratory Medicine, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou 510006, People’s Republic of China The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510006, People’s Republic of China Guangzhou University of Chinese Medicine, Guangzhou 510006, People’s Republic of China Department of Stomatology, Guangzhou Women and Children’s Medical Center, Guangzhou 510623, People’s Republic of China Present Address: Department of Laboratory Medicine, The Affiliated Hexian Memorial Hospital of Southern Medical University, Guangzhou, People’s Republic of China

    Article Title: SATB2-AS1 Suppresses Colorectal Carcinoma Aggressiveness by Inhibiting SATB2-Dependent Snail Transcription and Epithelial–Mesenchymal Transition
    Article Snippet: .. Briefly, total RNA was extracted from tissues and used to synthesize double-stranded cDNA. cDNA was labeled and hybridized to the LncRNA Expression Microarray (Arraystar, Rockville, MD). .. After hybridization and washing, slides were scanned with the Axon GenePix 4000B microarray scanner (Molecular Devices, Sunnyvale, CA).

    Article Title: SEMA3B‐AS1 suppresses colorectal carcinoma progression by inhibiting Semaphorin 3B‐dependent VEGF signaling pathway activation
    Article Snippet: .. LncRNA Expression Microarray (Arraystar) was used to label, purify, and hybridize the cDNA. .. An Agilent Microarray Scanner (Agilent p/n G2565BA) was used to scan slides after hybridization and washing.

    Article Title: Piplartine suppresses proliferation and invasion of hepatocellular carcinoma by LINC01391-modulated Wnt/β-catenin pathway inactivation through ICAT.
    Article Snippet: Accepted Manuscript Piplartine suppresses proliferation and invasion of hepatocellular carcinoma by LINC01391-modulated Wnt/β-catenin pathway inactivation through ICAT Xiaoxi Fan, Jingjing Song, Zhongwei Zhao, Minjiang Chen, Jianfei Tu, Chenying Lu, Fazong Wu, Dengke Zhang, Qiaoyou Weng, Liyun Zheng, Min Xu, Jiansong Ji PII: S0304-3835(19)30369-6 DOI: https://doi.org/10.1016/j.canlet.2019.06.008 Reference: CAN 114437 To appear in: Cancer Letters Received Date: 11 February 2019 Revised Date: 10 June 2019 Accepted Date: 11 June 2019 Please cite this article as: X.. Fan, J.. Song, Z. Zhao, M. Chen, J. Tu, C. Lu, F. Wu, D. Zhang, Q. Weng, L. Zheng, M. Xu, J. Ji, Piplartine suppresses proliferation and invasion of hepatocellular carcinoma by LINC01391-modulated Wnt/β-catenin pathway inactivation through ICAT, Cancer Letters, https:// doi.org/10.1016/j.canlet.2019.06.008.

    Article Title: Down-Regulation of Lnc-CYP7A1-1 Rejuvenates Aged Human Mesenchymal Stem Cells to Improve Their Efficacy for Heart Repair Through SYNE1
    Article Snippet: .. Total RNA was isolated from hBM-MSCs after cultured in hypoxic conditions for 72 h. 12 × 135K lncRNA Expression Microarray (Arraystar, Rockville, MD, United States) was used to detected hBM-MSCs cDNA. .. After hybridization, the processed slides were scanned by the Axon GenePix 4000B microarray scanner.

    Article Title: Alterations in the long non-coding RNA transcriptome in mesangial cells treated with aldosterone in vitro
    Article Snippet: Cells were cultured in 6-well plates for 24 h. MCs were washed with PBS three times, then 200 μl EDTA (Gibco; Thermo Fisher Scientific, Inc.) was added to each well followed by 750 μl TRIzol ® reagent (Invitrogen; Thermo Fisher Scientific, Inc.). .. The cells were stored at −80°C and sent to the LncRNA Expression Microarray (Arraystar, Rockville, MD, USA). .. The lncRNAs were constructed using public transcriptome databases (Refseq, www.ncbi.nlm.nih.gov/refseq ; University of California Santa Cruz knowngenes, genome.ucsc.edu ; and Gencode, www.gencodegenes.org ), in addition to a publication ( ).

    Article Title: <p>Long Non-Coding RNA DLEU1 Up-Regulates BIRC6 Expression by Competitively Sponging miR-381-3p to Promote Cisplatin Resistance in Nasopharyngeal Carcinoma</p>
    Article Snippet: Protein was normalized with β-actin (Cell Signaling Technology). .. Microarray Analysis Total RNA was extracted from three NPC tissues and three normal nasopharyngeal epithelial tissues using TRIzol submit your manuscript | www.dovepress.com DovePress OncoTargets and Therapy 2020:132038 O nc oT ar ge ts a nd T he ra py d ow nl oa de d fr om h ttp s: //w w w .d ov ep re ss .c om / b y 94 .2 31 .2 19 .7 3 on 2 7- M ar -2 02 0 F or p er so na l u se o nl y. Powered by TCPDF (www.tcpdf.org) 1 / 1 Reagent (Invitrogen) and purified by an RNeasy Mini Kit (Qiagen, Valencia, CA). cDNAwas synthesized, labeled, and hybridized to the LncRNA Expression Microarray (Arraystar, Rockville, MD). ..

    Article Title: Long Non-Coding RNA DLEU1 Up-Regulates BIRC6 Expression by Competitively Sponging miR-381-3p to Promote Cisplatin Resistance in Nasopharyngeal Carcinoma
    Article Snippet: Protein was normalized with β-actin (Cell Signaling Technology). .. Total RNA was extracted from three NPC tissues and three normal nasopharyngeal epithelial tissues using TRIzol Reagent (Invitrogen) and purified by an RNeasy Mini Kit (Qiagen, Valencia, CA). cDNA was synthesized, labeled, and hybridized to the LncRNA Expression Microarray (Arraystar, Rockville, MD). ..

    Isolation:

    Article Title: Down-Regulation of Lnc-CYP7A1-1 Rejuvenates Aged Human Mesenchymal Stem Cells to Improve Their Efficacy for Heart Repair Through SYNE1
    Article Snippet: .. Total RNA was isolated from hBM-MSCs after cultured in hypoxic conditions for 72 h. 12 × 135K lncRNA Expression Microarray (Arraystar, Rockville, MD, United States) was used to detected hBM-MSCs cDNA. .. After hybridization, the processed slides were scanned by the Axon GenePix 4000B microarray scanner.

    Cell Culture:

    Article Title: Down-Regulation of Lnc-CYP7A1-1 Rejuvenates Aged Human Mesenchymal Stem Cells to Improve Their Efficacy for Heart Repair Through SYNE1
    Article Snippet: .. Total RNA was isolated from hBM-MSCs after cultured in hypoxic conditions for 72 h. 12 × 135K lncRNA Expression Microarray (Arraystar, Rockville, MD, United States) was used to detected hBM-MSCs cDNA. .. After hybridization, the processed slides were scanned by the Axon GenePix 4000B microarray scanner.

    Purification:

    Article Title: <p>Long Non-Coding RNA DLEU1 Up-Regulates BIRC6 Expression by Competitively Sponging miR-381-3p to Promote Cisplatin Resistance in Nasopharyngeal Carcinoma</p>
    Article Snippet: Protein was normalized with β-actin (Cell Signaling Technology). .. Microarray Analysis Total RNA was extracted from three NPC tissues and three normal nasopharyngeal epithelial tissues using TRIzol submit your manuscript | www.dovepress.com DovePress OncoTargets and Therapy 2020:132038 O nc oT ar ge ts a nd T he ra py d ow nl oa de d fr om h ttp s: //w w w .d ov ep re ss .c om / b y 94 .2 31 .2 19 .7 3 on 2 7- M ar -2 02 0 F or p er so na l u se o nl y. Powered by TCPDF (www.tcpdf.org) 1 / 1 Reagent (Invitrogen) and purified by an RNeasy Mini Kit (Qiagen, Valencia, CA). cDNAwas synthesized, labeled, and hybridized to the LncRNA Expression Microarray (Arraystar, Rockville, MD). ..

    Article Title: Long Non-Coding RNA DLEU1 Up-Regulates BIRC6 Expression by Competitively Sponging miR-381-3p to Promote Cisplatin Resistance in Nasopharyngeal Carcinoma
    Article Snippet: Protein was normalized with β-actin (Cell Signaling Technology). .. Total RNA was extracted from three NPC tissues and three normal nasopharyngeal epithelial tissues using TRIzol Reagent (Invitrogen) and purified by an RNeasy Mini Kit (Qiagen, Valencia, CA). cDNA was synthesized, labeled, and hybridized to the LncRNA Expression Microarray (Arraystar, Rockville, MD). ..

    Synthesized:

    Article Title: <p>Long Non-Coding RNA DLEU1 Up-Regulates BIRC6 Expression by Competitively Sponging miR-381-3p to Promote Cisplatin Resistance in Nasopharyngeal Carcinoma</p>
    Article Snippet: Protein was normalized with β-actin (Cell Signaling Technology). .. Microarray Analysis Total RNA was extracted from three NPC tissues and three normal nasopharyngeal epithelial tissues using TRIzol submit your manuscript | www.dovepress.com DovePress OncoTargets and Therapy 2020:132038 O nc oT ar ge ts a nd T he ra py d ow nl oa de d fr om h ttp s: //w w w .d ov ep re ss .c om / b y 94 .2 31 .2 19 .7 3 on 2 7- M ar -2 02 0 F or p er so na l u se o nl y. Powered by TCPDF (www.tcpdf.org) 1 / 1 Reagent (Invitrogen) and purified by an RNeasy Mini Kit (Qiagen, Valencia, CA). cDNAwas synthesized, labeled, and hybridized to the LncRNA Expression Microarray (Arraystar, Rockville, MD). ..

    Article Title: Long Non-Coding RNA DLEU1 Up-Regulates BIRC6 Expression by Competitively Sponging miR-381-3p to Promote Cisplatin Resistance in Nasopharyngeal Carcinoma
    Article Snippet: Protein was normalized with β-actin (Cell Signaling Technology). .. Total RNA was extracted from three NPC tissues and three normal nasopharyngeal epithelial tissues using TRIzol Reagent (Invitrogen) and purified by an RNeasy Mini Kit (Qiagen, Valencia, CA). cDNA was synthesized, labeled, and hybridized to the LncRNA Expression Microarray (Arraystar, Rockville, MD). ..



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    The regulatory mechanism of the competitive endogenous RNA networks in transcription, translation, and epigenetics . <t>LncRNA</t> regulates transcription by splicing and degrading RNA, while posttranscriptional regulation is controlled by decoy and sponge proteins, as well as epigenetic modification. CircRNA regulates transcription and translation by binding to miRNA and interacting with RNA-binding protein, particularly in certain circRNA can code protein. Both lncRNA and circRNA can serve as competitive endogenous RNA that bind with miRNA to regulate the translation of targeted mRNA, thereby inhibiting mRNA translation and promoting mRNA degradation. See text for details. circRNA, circular RNA; lncRNA, long non-coding RNA; miRNA, microRNA; mRNA, messenger RNA; UTR, untranslated regions.
    Human Gene Expression Microarray Arraystar Human 8 × 60 K Lncrna + Mrna Microarray V2.0, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Long noncoding RNA ( LncRNA) SEMA3B‐AS1 could be an important prognostic factor in predicting the survival of colorectal carcinoma (CRC) patients . (A) Hierarchical clustering analysis of differentially expressed lncRNAs that intersected between CRC tissues and paracancerous normal intestinal mucosal tissues, as well as between CRC cells SW620 and SW480. N, normal intestinal mucosa tissues. C, colorectal carcinoma tissues. The red square highlights SEMA3B‐AS1 . (B) The level of SEMA3B‐AS1 in paired colorectal carcinoma and adjacent noncancerous tissues. (C) The level of SEMA3B‐AS1 in colorectal carcinoma and colon mucosa epithelial (FHC) cell lines. (D) Expression analysis of SEMA3B‐AS1 in normal colorectal mucosa and colorectal carcinoma tissues by in situ hybridization (ISH). Scale bars are shown in the right–left corner of each picture. The scale bar indicates 100 μm in upper row, and the scale bar indicates 20 μm in lower row. (E) Correlation among TNM, lymph node metastasis, distant metastasis, and expression of SEMA3B‐AS1 . (F and G) Kaplan–Meier survival analysis in all patients with colorectal carcinoma according to SEMA3B‐AS1 expression according to our data (F) and The Cancer Genome Atlas (TCGA) cohort (G). For (B)–(E), data are presented as means ± SD in three independent experiments. * p < 0.05; ** p < 0.01; *** p < 0.001.

    Journal: MedComm

    Article Title: SEMA3B‐AS1 suppresses colorectal carcinoma progression by inhibiting Semaphorin 3B‐dependent VEGF signaling pathway activation

    doi: 10.1002/mco2.365

    Figure Lengend Snippet: Long noncoding RNA ( LncRNA) SEMA3B‐AS1 could be an important prognostic factor in predicting the survival of colorectal carcinoma (CRC) patients . (A) Hierarchical clustering analysis of differentially expressed lncRNAs that intersected between CRC tissues and paracancerous normal intestinal mucosal tissues, as well as between CRC cells SW620 and SW480. N, normal intestinal mucosa tissues. C, colorectal carcinoma tissues. The red square highlights SEMA3B‐AS1 . (B) The level of SEMA3B‐AS1 in paired colorectal carcinoma and adjacent noncancerous tissues. (C) The level of SEMA3B‐AS1 in colorectal carcinoma and colon mucosa epithelial (FHC) cell lines. (D) Expression analysis of SEMA3B‐AS1 in normal colorectal mucosa and colorectal carcinoma tissues by in situ hybridization (ISH). Scale bars are shown in the right–left corner of each picture. The scale bar indicates 100 μm in upper row, and the scale bar indicates 20 μm in lower row. (E) Correlation among TNM, lymph node metastasis, distant metastasis, and expression of SEMA3B‐AS1 . (F and G) Kaplan–Meier survival analysis in all patients with colorectal carcinoma according to SEMA3B‐AS1 expression according to our data (F) and The Cancer Genome Atlas (TCGA) cohort (G). For (B)–(E), data are presented as means ± SD in three independent experiments. * p < 0.05; ** p < 0.01; *** p < 0.001.

    Article Snippet: LncRNA Expression Microarray (Arraystar) was used to label, purify, and hybridize the cDNA.

    Techniques: Expressing, In Situ Hybridization

    The regulatory mechanism of the competitive endogenous RNA networks in transcription, translation, and epigenetics . LncRNA regulates transcription by splicing and degrading RNA, while posttranscriptional regulation is controlled by decoy and sponge proteins, as well as epigenetic modification. CircRNA regulates transcription and translation by binding to miRNA and interacting with RNA-binding protein, particularly in certain circRNA can code protein. Both lncRNA and circRNA can serve as competitive endogenous RNA that bind with miRNA to regulate the translation of targeted mRNA, thereby inhibiting mRNA translation and promoting mRNA degradation. See text for details. circRNA, circular RNA; lncRNA, long non-coding RNA; miRNA, microRNA; mRNA, messenger RNA; UTR, untranslated regions.

    Journal: Reviews in Cardiovascular Medicine

    Article Title: Revealing Landscape of Competing Endogenous RNA Networks in Sepsis-Induced Cardiovascular Diseases

    doi: 10.31083/j.rcm2407214

    Figure Lengend Snippet: The regulatory mechanism of the competitive endogenous RNA networks in transcription, translation, and epigenetics . LncRNA regulates transcription by splicing and degrading RNA, while posttranscriptional regulation is controlled by decoy and sponge proteins, as well as epigenetic modification. CircRNA regulates transcription and translation by binding to miRNA and interacting with RNA-binding protein, particularly in certain circRNA can code protein. Both lncRNA and circRNA can serve as competitive endogenous RNA that bind with miRNA to regulate the translation of targeted mRNA, thereby inhibiting mRNA translation and promoting mRNA degradation. See text for details. circRNA, circular RNA; lncRNA, long non-coding RNA; miRNA, microRNA; mRNA, messenger RNA; UTR, untranslated regions.

    Article Snippet: A total of 30,584 differentially expressed lncRNAs (1068 downregulated and 871 upregulated) were screened by the Arraystar Human lncRNA Expression Microarray; among them, CTC-459I6.1 and AL132709.5 were the most downregulated and upregulated lncRNAs, respectively.

    Techniques: Modification, Binding Assay, RNA Binding Assay

    The lncRNA associated ceRNA networks in sepsis-induced cardiotoxicity .

    Journal: Reviews in Cardiovascular Medicine

    Article Title: Revealing Landscape of Competing Endogenous RNA Networks in Sepsis-Induced Cardiovascular Diseases

    doi: 10.31083/j.rcm2407214

    Figure Lengend Snippet: The lncRNA associated ceRNA networks in sepsis-induced cardiotoxicity .

    Article Snippet: A total of 30,584 differentially expressed lncRNAs (1068 downregulated and 871 upregulated) were screened by the Arraystar Human lncRNA Expression Microarray; among them, CTC-459I6.1 and AL132709.5 were the most downregulated and upregulated lncRNAs, respectively.

    Techniques: Biomarker Discovery, Diagnostic Assay, Activity Assay, Expressing, Inhibition, Migration, Membrane

    The lncRNA associated ceRNA networks of septic cardiovascular toxicity in non-cardiomyocytes .

    Journal: Reviews in Cardiovascular Medicine

    Article Title: Revealing Landscape of Competing Endogenous RNA Networks in Sepsis-Induced Cardiovascular Diseases

    doi: 10.31083/j.rcm2407214

    Figure Lengend Snippet: The lncRNA associated ceRNA networks of septic cardiovascular toxicity in non-cardiomyocytes .

    Article Snippet: A total of 30,584 differentially expressed lncRNAs (1068 downregulated and 871 upregulated) were screened by the Arraystar Human lncRNA Expression Microarray; among them, CTC-459I6.1 and AL132709.5 were the most downregulated and upregulated lncRNAs, respectively.

    Techniques: Biomarker Discovery, Expressing

    LncRNA-mediated competitive endogenous RNA networks in septic cardiomyopathy . LncRNAs CRNDE, MIAT , and SNHG1 are associated with septic cardiomyopathy by regulating oxidative stress. LncRNAs H19, RMRP , and TTN-AS1 are associated with septic cardiomyopathy by regulating mitochondrial injury. Yin Yang-1 and matrine alleviate septic cardiomyopathy by upregulating LINC00472 and downregulating PTENP1 , respectively. Palmitic acid and saturated fatty acid aggravate septic cardiomyopathy by upregulating GAS5 and MALAT1 , respectively. The up-arrow indicates upregulation and the down-arrow indicates downregulation. The arrow-line represents promotion and the T-line represents inhibition. ANRIL, antisense ncRNA in the INK4 locus; CRNDE , colorectal neoplasia differentially expressed; E2F2, E2F transcription factor 2; GAS5 , growth arrest specific 5; H19 , H19 imprinted maternally expressed transcript; HMGB1, high mobility group protein 1; HSPA4, heat shock 70 kDa protein 4; IL-6, interleukin-6; MALAT1 , metastasis associated lung adenocarcinoma transcript 1; MAOA, monoamine oxidase A; MIAT, myocardial infarction associated transcript; NF- κ B, nuclear factor- κ B; PTENP1 , PTEN pseudogene-1; RMRP , RNA component of mitochondrial RNA processing endoribonuclease; SIRT1, sirtuins 1; SNHG16 , small nucleolar RNA host gene 16; SORBS2, sorbin and SH3 domain‐containing 2; TLR4, toll-like receptor 4; TRAF6, TNF receptor associated factor; TTN-AS1 , TTN antisense RNA 1; XIAP, X-linked inhibitor of apoptosis gene; lncRNA, long non-coding RNA.

    Journal: Reviews in Cardiovascular Medicine

    Article Title: Revealing Landscape of Competing Endogenous RNA Networks in Sepsis-Induced Cardiovascular Diseases

    doi: 10.31083/j.rcm2407214

    Figure Lengend Snippet: LncRNA-mediated competitive endogenous RNA networks in septic cardiomyopathy . LncRNAs CRNDE, MIAT , and SNHG1 are associated with septic cardiomyopathy by regulating oxidative stress. LncRNAs H19, RMRP , and TTN-AS1 are associated with septic cardiomyopathy by regulating mitochondrial injury. Yin Yang-1 and matrine alleviate septic cardiomyopathy by upregulating LINC00472 and downregulating PTENP1 , respectively. Palmitic acid and saturated fatty acid aggravate septic cardiomyopathy by upregulating GAS5 and MALAT1 , respectively. The up-arrow indicates upregulation and the down-arrow indicates downregulation. The arrow-line represents promotion and the T-line represents inhibition. ANRIL, antisense ncRNA in the INK4 locus; CRNDE , colorectal neoplasia differentially expressed; E2F2, E2F transcription factor 2; GAS5 , growth arrest specific 5; H19 , H19 imprinted maternally expressed transcript; HMGB1, high mobility group protein 1; HSPA4, heat shock 70 kDa protein 4; IL-6, interleukin-6; MALAT1 , metastasis associated lung adenocarcinoma transcript 1; MAOA, monoamine oxidase A; MIAT, myocardial infarction associated transcript; NF- κ B, nuclear factor- κ B; PTENP1 , PTEN pseudogene-1; RMRP , RNA component of mitochondrial RNA processing endoribonuclease; SIRT1, sirtuins 1; SNHG16 , small nucleolar RNA host gene 16; SORBS2, sorbin and SH3 domain‐containing 2; TLR4, toll-like receptor 4; TRAF6, TNF receptor associated factor; TTN-AS1 , TTN antisense RNA 1; XIAP, X-linked inhibitor of apoptosis gene; lncRNA, long non-coding RNA.

    Article Snippet: A total of 30,584 differentially expressed lncRNAs (1068 downregulated and 871 upregulated) were screened by the Arraystar Human lncRNA Expression Microarray; among them, CTC-459I6.1 and AL132709.5 were the most downregulated and upregulated lncRNAs, respectively.

    Techniques: Inhibition

    LncRNA-mediated ceRNA networks in vascular endothelial cells and mononuclear macrophages under sepsis environment . LncRNAs LUADT1, HULC , and SNHG15 are involved in sepsis endothelial dysfunction. LncRNAs PVT1, HOTAR , and SNHG16 are involved in sepsis-induced macrophage polarization. In general, lncRNA MALAT1 aggravates sepsis-induced endothelial dysfunction and macrophage polarization, but MALAT1 inhibits LPS-induced RAW264.7 macrophage apoptosis by regulating has-miR-346/SMAD3 axis. The up-arrow indicates upregulation and the down-arrow indicates downregulation. The arrow-line represents promotion and the T-line represents inhibition. HMGB1, high mobility group protein 1; HOTAIR , hox transcript antisense RNA; HULC, highly upregulated in liver cancer; IL-6, interleukin-6; LUADT1, lung adenocarcinoma transcript 1; MALAT1 , metastasis associated lung adenocarcinoma transcript 1; MCEMP1, mast cell expressed membrane protein 1; NF- κ B, nuclear factor- κ B; PIM-1, pim-1 proto-oncogene; PVT1 , plasmacytoma variant translocation 1; SMAD3, SMAD family member 3; SNHG15/16 , small nucleolar RNA host gene 15/16; TLR4, toll-like receptor 4; TRPM7, transient receptor potential melastatin-subfamily member 7; lncRNA, long non-coding RNA.

    Journal: Reviews in Cardiovascular Medicine

    Article Title: Revealing Landscape of Competing Endogenous RNA Networks in Sepsis-Induced Cardiovascular Diseases

    doi: 10.31083/j.rcm2407214

    Figure Lengend Snippet: LncRNA-mediated ceRNA networks in vascular endothelial cells and mononuclear macrophages under sepsis environment . LncRNAs LUADT1, HULC , and SNHG15 are involved in sepsis endothelial dysfunction. LncRNAs PVT1, HOTAR , and SNHG16 are involved in sepsis-induced macrophage polarization. In general, lncRNA MALAT1 aggravates sepsis-induced endothelial dysfunction and macrophage polarization, but MALAT1 inhibits LPS-induced RAW264.7 macrophage apoptosis by regulating has-miR-346/SMAD3 axis. The up-arrow indicates upregulation and the down-arrow indicates downregulation. The arrow-line represents promotion and the T-line represents inhibition. HMGB1, high mobility group protein 1; HOTAIR , hox transcript antisense RNA; HULC, highly upregulated in liver cancer; IL-6, interleukin-6; LUADT1, lung adenocarcinoma transcript 1; MALAT1 , metastasis associated lung adenocarcinoma transcript 1; MCEMP1, mast cell expressed membrane protein 1; NF- κ B, nuclear factor- κ B; PIM-1, pim-1 proto-oncogene; PVT1 , plasmacytoma variant translocation 1; SMAD3, SMAD family member 3; SNHG15/16 , small nucleolar RNA host gene 15/16; TLR4, toll-like receptor 4; TRPM7, transient receptor potential melastatin-subfamily member 7; lncRNA, long non-coding RNA.

    Article Snippet: A total of 30,584 differentially expressed lncRNAs (1068 downregulated and 871 upregulated) were screened by the Arraystar Human lncRNA Expression Microarray; among them, CTC-459I6.1 and AL132709.5 were the most downregulated and upregulated lncRNAs, respectively.

    Techniques: Inhibition, Membrane, Variant Assay, Translocation Assay

    Apoptosis and pyroptosis associated competitive endogenous RNA networks in septic cardiomyopathy . LncRNAs CYTOR, GAS5, KCNQ1OT1, LUCAT1 , and NEAT1 are associated with septic cardiomyopathy by regulating apoptosis. LncRNAs XIST and ZFAS1 are associated with septic cardiomyopathy by regulating pyroptosis. The up-arrow indicates upregulation and the down-arrow indicates downregulation. The arrow-line represents promotion and the T-line represents inhibition. c-Fos, A nuclear phosphoprotein; CYTOR , cytoskeleton regulator RNA; GAS5 , growth arrest specific 5; KCNQ1OT1 , KCNQ1 opposite strand/antisense transcript 1; LUCAT1 , lung cancer-related transcript 1; NEAT1 , nuclear paraspeckle assembly transcript 1; NF- κ B, nuclear factor- κ B; NLRP3, NOD-like receptor thermal protein domain associated protein 3; PGC-1α, peroxisome proliIerators-activated receptor γ coactivator l alpha; ROCK1, rho associated coiled-coil containing protein kinase 1; SESN2, sestrin 2; SIRT1, silent information regulator 1; XIAP, X-linked inhibitor of apoptosis gene; XIST , X-inactive specific transcript; ZFAS1 , zinc finger antisense 1; lncRNA, long non-coding RNA.

    Journal: Reviews in Cardiovascular Medicine

    Article Title: Revealing Landscape of Competing Endogenous RNA Networks in Sepsis-Induced Cardiovascular Diseases

    doi: 10.31083/j.rcm2407214

    Figure Lengend Snippet: Apoptosis and pyroptosis associated competitive endogenous RNA networks in septic cardiomyopathy . LncRNAs CYTOR, GAS5, KCNQ1OT1, LUCAT1 , and NEAT1 are associated with septic cardiomyopathy by regulating apoptosis. LncRNAs XIST and ZFAS1 are associated with septic cardiomyopathy by regulating pyroptosis. The up-arrow indicates upregulation and the down-arrow indicates downregulation. The arrow-line represents promotion and the T-line represents inhibition. c-Fos, A nuclear phosphoprotein; CYTOR , cytoskeleton regulator RNA; GAS5 , growth arrest specific 5; KCNQ1OT1 , KCNQ1 opposite strand/antisense transcript 1; LUCAT1 , lung cancer-related transcript 1; NEAT1 , nuclear paraspeckle assembly transcript 1; NF- κ B, nuclear factor- κ B; NLRP3, NOD-like receptor thermal protein domain associated protein 3; PGC-1α, peroxisome proliIerators-activated receptor γ coactivator l alpha; ROCK1, rho associated coiled-coil containing protein kinase 1; SESN2, sestrin 2; SIRT1, silent information regulator 1; XIAP, X-linked inhibitor of apoptosis gene; XIST , X-inactive specific transcript; ZFAS1 , zinc finger antisense 1; lncRNA, long non-coding RNA.

    Article Snippet: A total of 30,584 differentially expressed lncRNAs (1068 downregulated and 871 upregulated) were screened by the Arraystar Human lncRNA Expression Microarray; among them, CTC-459I6.1 and AL132709.5 were the most downregulated and upregulated lncRNAs, respectively.

    Techniques: Inhibition

    The lncRNA- and circRNA-associated competitive endogenous RNA networks in septic cardiovascular dysfunction . The regulatory mechanisms and potential functions of ceRNA networks in septic cardiomyopathy and vascular paralysis by regulating inflammation, oxidative response, endothelial dysfunction, macrophage polarization, apoptosis and pyroptosis, along with metabolic energy impairment and endoplasmic reticulum stress. See text for details.

    Journal: Reviews in Cardiovascular Medicine

    Article Title: Revealing Landscape of Competing Endogenous RNA Networks in Sepsis-Induced Cardiovascular Diseases

    doi: 10.31083/j.rcm2407214

    Figure Lengend Snippet: The lncRNA- and circRNA-associated competitive endogenous RNA networks in septic cardiovascular dysfunction . The regulatory mechanisms and potential functions of ceRNA networks in septic cardiomyopathy and vascular paralysis by regulating inflammation, oxidative response, endothelial dysfunction, macrophage polarization, apoptosis and pyroptosis, along with metabolic energy impairment and endoplasmic reticulum stress. See text for details.

    Article Snippet: A total of 30,584 differentially expressed lncRNAs (1068 downregulated and 871 upregulated) were screened by the Arraystar Human lncRNA Expression Microarray; among them, CTC-459I6.1 and AL132709.5 were the most downregulated and upregulated lncRNAs, respectively.

    Techniques: